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Tag Archives: siRNA

MicroRNA and siRNA Cloning Method

1. Oligo Design 2. ImpA Synthesis 3. Synthesizing Oligos 4. Oligonucleotide Adenylation 5. Small RNA Purification 6. 3′ Adaptor Ligation 7. 5′ Adaptor Ligation 8. RT-PCR 9. Concatamerization 10. Cloning into Topo vector 11. Screen and Sequence…

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A protocol for designing siRNAs with high functionality and specificity

Effective gene silencing by the RNA interference (RNAi) pathway requires a comprehensive understanding of the elements that influence small interfering RNA (siRNA) functionality and specificity. These include (i) sequence space restrictions that define the boundaries of siRNA targeting, (ii) structural and sequence features required for efficient siRNA performance, (iii) mechanisms that underlie nonspecific gene modulation…

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