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Tag Archives: Phosphorylation Assay

Phosphorylation Assay

Materials required: Cells PBS HAM F-12 media Growth factor (VEGF) Lysis Buffer mix (w/o PMSF and Aprotinin, keep at 4C, add PMSF and Aprotinin right before use): 1% Triton-X100 10mM Tris 1mM EDTA 150mM NaCl 30mM PPiNa 50mM NaFi 2.1mM Na3VO4…

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