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Tag Archives: Phalloidin staining

Phalloidin staining of F-actin in embryos

Grow up worms to gravid adult stage on high density or NGM plates. Wash off worms with M9. Add 10 ml M9 to the plates and use a rubber policeman to gently dislodge the eggs from the plates. Pipette eggs into 15 ml tubes. If you want earlier stage eggs, save adults and bleach, but…

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