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Tag Archives: PCR

Real Time PCR Protocols

Real Time PCR vs. Traditional PCR Real Time PCR: A must read material Principal of Real Time PCR (60 slideshows) Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines Description Protocol 1. Quantitative Real Time PCR, a Practical Tutorial Protocol 2. Real time quantitative RT-PCR (Taqman) Protocol 3. TAQurate? GREEN Real-Time PCR MasterMix Real-time PCR complete solutions Protocol 4. Real…

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PCR Protocol

PCR(Polymerase Chain Reaction) is a basic biological technique which amplify double strain DNA by magnitudes. Preparation Thermal cycler(also called PCR machine, DNA amplifier), PCR tubes(200μl capacity), DNA polymerase, dNTP, DNA templates, DNA primers. PCR Protocol 1.Mix the DNA polymerase, dNTP, DNA templates and DNA primers in a clean PCR tube. Then put the tube in…

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Lab-manual.com — Bioprotocols online

General Lab Techniques www.lab-manual.com/lm_280.htm Lab security and basic techniques Advanced lab skills   Molecular Separation & Labeling www.lab-manual.com/lm_280.htm Molecular Separation Molecular Tracer Labeling   DNA and RNA www.lab-manual.com/lm_281.htm Nucleic acid methods (1) Microarray protocols.   Genetics www.lab-manual.com/lm_282.htm Epigenetics protocols Single nucleotide polymorphisms (SNPs)   Molecular Biology–PCR Serials www.lab-manual.com/lm_282.htm PCR,RT-PCR,Real time PCR etc. PCR (General Procedure)   Molecular Biology–Protein www.lab-manual.com/lm_283.htm Protein methods Western blot hybridization  …

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Agglutination-PCR

Agglutination-PCR (ADAP) is an ultrasensitive solution-phase method for detecting antibodies. Antibodies bind to and agglutinate synthetic antigen–DNA conjugates, enabling ligation of the DNA strands and subsequent quantification by qPCR. Like other Immuno-PCR (IPCR) detection methods ADAP combines the specificity of antibody-antigen recognition and the sensitivity of PCR. ADAP detects zepto- to attomoles of antibodies in 2…

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