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Tag Archives: Mitochondrial DNA Isolation

Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing

Abstract: Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequencing results. Here, we describe a fast, cost-effective, and reliable method for preparation of mtDNA enriched samples from eukaryotic cells ready for direct…

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Mitochondrial DNA Isolation

What’s mitochondrial DNA? mitochondrial DNA (mtDNA) is a genetic material of the mitochondria, the organelles that generate energy for the cell. In mammals, mtDNA makes up less than 1% of the total cellular DNA, but in plants the amount is variable. It codes for ribosomal RNA and transfer RNA, but only some mitochondrial proteins (up to 30…

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