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Tag Archives: Manual Protocol

Hematoxylin and Eosin (H & E) Staining

•Place slides containing paraffin sections in a slide holder (glass or metal) •Deparaffinize and rehydrate sections: 3 x 3´ Xylene (blot excess xylene before going into ethanol) 3 x 3´ 100% ethanol 1 x 3´ 95% ethanol 1 x 3´ 80% ethanol 1 x 5´ deionized H2O •While sections are in water, skim surface of…

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