Tag Archives: Elisa

Elisa blocking buffer and coating buffer recipes

ELISA Blocking Buffer Dissolve 5.3g of Na2CO3 in 900ml distilled H2O Dissolve 4.2g of NaHCO3 in the solution from step 1 Dissolve 1g sodium azide in the solution from step 2 Dissolve 5g BSA in solution from step 3 Alternatively you can also use 50g non-fat dry milk for blocking solution pH to 9.6 Adjust volume to…

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