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Tag Archives: E. coli Miniprep

E. coli Miniprep

1) Grow 2ml culture o/n. 2) Spin down. Pour off all but ~100ul of sup. Resuspend cells in the ~100ul. 3) Add 130ul P1. Resuspend. 4) Add 130ul P2. Mix immediately. 5) Add 182ul N3. Mix immediately. 6) Spin 10 min at 4C. (Removes genomic DNA and debris). 7) Transfer sup to a fresh eppendorf.…

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