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Tag Archives: Colony Formation

Soft Agar Assay for Colony Formation

  Note: All volumes are calculated to cater for four plates per point. Base Agar Melt 1% Agar (DNA grade) in microwave, cool to 40C in a waterbath. Warm 2X RPMI + 20% FCS to 40C in waterbath. Allow at least 30 minutes for temperature to equilibrate. Mix equal volumes of the two solutions to…

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