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Category Archives: Biology

Biology

Most recent 10 Nobel Prizes for medicine or physiology

2018-  James P Allison and Tasuku Honjo for Immune checkpoint therapy to treatments for advanced, deadly cancers. 2017- Jeffrey Hall, Michael Rosbash and Michael Young for unravelling how bodies keep a circadian rhythm or body clock 2016 – Yoshinori Ohsumi for discovering how cells remain healthy by recycling waste. 2015 – William C Campbell, Satoshi Ōmura and…

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2018 Nobel prize for medicine goes to cancer immunotherapy

Two scientists who discovered how to fight cancer using the body’s immune system have won the 2018 Nobel Prize for physiology or medicine. The work, by Professor James P Allison from the US and Professor Tasuku Honjo from Japan, has led to treatments for advanced, deadly skin cancer. Immune checkpoint therapy has revolutionised cancer treatment,…

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Cancer Stem Cell Theory

Research has shown that cancer cells are not all the same. Within a malignant tumor or among the circulating cancerous cells of a leukemia, there can be a variety of types of cells. The stem cell theory of cancer proposes that among all cancerous cells, a few act as stem cells that reproduce themselves and…

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T Cells Isolation and Purification

Remove the spleen. Grind the spleen with the flat end of a syringe in 5 ml of RPMI on a 100 mm culture dish. Pass through all cells through a cell strainer into a 50 ml tube. Wash the cell culture dish with 5 ml of RPMI twice and combine all cells. Spin down the cells and remove…

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PCR Based Moleclar Cloning

In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can be easily cloned into a plasmid of interest. You can use similar processes to add overhangs to your…

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