Phalloidin staining of F-actin in embryos

Grow up worms to gravid adult stage on high density or NGM plates. Wash off worms with M9. Add 10 ml M9 to the plates and use a rubber policeman to gently dislodge the eggs from the plates. Pipette eggs into 15 ml tubes. If you want earlier stage eggs, save adults and bleach, but…

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How To Make a Model of the Lungs

Constructing a lung model is an excellent way to learn about the respiratory systemand how the lungs function. The lungs are organs that provide a place for gas exchange between air from the outside environment and gasses in the blood. Gas exchange occurs at lung alveoli (tiny air sacs) as carbon dioxide is exchanged for oxygen. Breathing is controlled by a…

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Isopropanol precipitation of DNA

Alcohol precipitation is commonly used for concentrating, desalting, and recovering nucleic acids. Precipitation is mediated by high concentrations of salt and the addition of either isopropanol or ethanol. Since less alcohol is required for isopropanol precipitation, this is the preferred method for precipitating DNA from large volumes. In addition, isopropanol precipitation can be performed at…

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GeneTalk is a web-based platform, tool, and database, for filtering, reduction and prioritization of human sequence variants from next-generation sequencing (NGS) data. GeneTalk allows editing annotation about sequence variants and build up a crowd sourced database with clinically relevant information for diagnostics of genetic disorders. GeneTalk allows searching for information about specific sequence variants and connects to experts on variants that are potentially disease-relevant. Application…

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Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells

The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-free dissociation buffers compared to trypsin. Subsequently, the dissociated cells were re-seeded on new cell culture dishes and were subjected to the MTT assay 24 h later. The proportion…

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