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Biorptocol: DNA Modification/Epigenetics

Analysis of Mitotic Checkpoint Function in Xenopus Egg Extracts Yinghui Mao Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot099853 Abstract  Full Text (PDF)   Analysis of DNA Methylation in Mammalian Cells Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee Cold Spring Harb Protoc 2017; doi:10.1101/pdb.top094821 Abstract  Full Text  Full Text (PDF)   Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection Paul M. Lizardi, Qin Yan, and Narendra…

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Trump-Russia: Flynn’s dealings were ‘lawful’

Donald Trump has responded to a guilty plea by his former national security adviser Michael Flynn, saying Mr Flynn’s actions as a member of his transition team “were lawful”. Mr Flynn has entered a plea deal and agreed to co-operate with an inquiry into alleged collusion with Russia. The deal, for a lesser charge than…

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Plesk General Error: 1021 Disk full (/var/tmp/#sql_b02b_0); waiting for…

ERROR: Plesk\Exception\Database: DB query failed: SQLSTATE[HY000]: General error: 1021 Disk full (/var/tmp/#sql_b02b_0); waiting for someone to free some space…, query was: DESCRIBE `sessions` Additionally, an exception has occurred while trying to report this error: Zend_Exception No entry is registered for key ‘translate’ (Mysql.php:53) Cause The file /var/lock/lmlib/container_locks/authorizer.lock is corrupted. Resolution Perform the following steps to fix the…

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Total DNA isolation protocol

The procedure is suitable for all types of tissues from wide variety of animal, blood and plant species. All DNA extraction steps are performed at weak acid pH (HEPES free acid) and optionally with hot chloroform for ‘difficult’ samples, and at room temperature. The following protocol is designed for small and large tissue samples (tissue…

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