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2.5 M CaCl2 (20 ml)

  1. Weigh out 13.5 g CaCl2?H2O and dissolve in 15 ml of distilled water.
  2. Make up volume to 20 ml with distilled water.
  3. Sterilize the solution by filtration through a 0.22 micron filter.
    <STORAGE> Store in 1 ml aliquots at -20 C.

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