1 M CaCl2 (200 ml)

  1. Weigh out 54 g CaCl2?H2O and dissolve in 170 ml of distilled water.
  2. Make up volume to 200 ml with distilled water.
  3. Sterilize the solution by passage through a 0.22-micron filter.
    <STORAGE> Store in 1 ml aliquots at -20 C.
    <USAGE> When preparing competent cells, thaw an aliquot and dilute it to 100ml with distilled water. Sterilize the solution by filtration through a Nalgene filter (0.45 micron), and chill it to 0 C before use.

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