0.1 M Adenosine triphosphate (ATP)(1 ml)

  1. Weigh out 60 mg ATP.
  2. Add 0.8 ml distilled water.
  3. Adjust to pH 7.0 with 0.1 M NaOH.
  4. Make up volume to 1 ml with distilled water.
    <STORAGE> Aliquots of appropriate volume should be stored at -70 C.

10 M Ammonium acetate (1 liter)

  1. Weigh out 770 g ammonium acetate.
  2. Add 800 ml distilled water to dissolve.
  3. Make up volume to 1 liter with distilled water.
  4. Sterilize by filtration.
    <CRITICAL> Ammonium acetate is heat labile. Do not autoclave.
    <STORAGE> Store at 4 C.

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