Animal’s lower left abdominal quadrant.
21 gauge or smaller (usually use 25 gauge for luciferin injection)
100ul of luciferin (15mg/ml stock) per 10 grams of mouse body weight.
Note: 1ml i.p. injection of a nonirritating solution is easily tolerated.
Position of animal:
Manually restrained, dorsal recumbency (abdomen side up), with cranial (head)
end of animal pointed down. Gently shake the mouse 2-3 times to encourage the
intestinal content downward and to create an empty cavity in the lower
abdominal quadrant. Prep the site with 70% ethanol.
Needle should be bevel-side up and slightly angled 15 – 20 degrees when
entering the abdominal cavity. Penetrate just through abdominal wall (about 4-