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Tag Archives: T Cells Isolation and Purification

T Cells Isolation and Purification

Remove the spleen. Grind the spleen with the flat end of a syringe in 5 ml of RPMI on a 100 mm culture dish. Pass through all cells through a cell strainer into a 50 ml tube. Wash the cell culture dish with 5 ml of RPMI twice and combine all cells. Spin down the cells and remove…

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