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Tag Archives: RNA Amplification

Protocol of RNA Amplification

First strand synthesis The standard protocol for amplifications employed a 10 µl reverse transcription (RT) reaction with 200 U SuperScript III (Invitrogen), I µl of linear acryl amide (0.1µg/µl, Ambion, ….) and 1 µg T7-N6s:  5’AATTGTAATACGACTCACTATAGGGNNNNN[N-Q]  in 1x first-strand buffer (Life Technologies) with a 50°C incubation. Add the following RNA template (100 ng) 1 ug…

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