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Tag Archives: protein Gels

Silver Staining of Gels

Materials Protein gel 45% (v/v) Methanol + 12% (w/v) acetic acid 5% (v/v) Methanol + 7% (w/v) acetic acid 10% Glutaraldehyde 0.01M Dithiothreitol Silver nitrate solution Sodium citrate / formaldehyde Kodak Farmer’s Reducer or Kodak Rapid Fixer Procedure Fix gels by gently rocking them in a solution of 45% methanol / 12% acetic acid until…

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