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Tag Archives: Miniprep DNA isolation

Miniprep double-stranded DNA isolation

The standard method for the miniprep isolation of plasmid DNA includes the same general strategy as the large scale isolation. However, smaller aliquots of antibiotic containing liquid media inoculated with plasmid-containing cell colonies are incubated in a 37degC shaker for 12-16 hours. After collecting the plasmid containing cells by centrifugation, the cell pellet is resuspended…

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