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Tag Archives: MicroRNA

MicroRNA and siRNA Cloning Method

1. Oligo Design 2. ImpA Synthesis 3. Synthesizing Oligos 4. Oligonucleotide Adenylation 5. Small RNA Purification 6. 3′ Adaptor Ligation 7. 5′ Adaptor Ligation 8. RT-PCR 9. Concatamerization 10. Cloning into Topo vector 11. Screen and Sequence…

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