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Tag Archives: Intraperitoneal Injections

Proper Method for Intraperitoneal Injections

Preferred site: Animal’s lower left abdominal quadrant. Needle size: 21 gauge or smaller (usually use 25 gauge for luciferin injection) Volume: 100ul of luciferin (15mg/ml stock) per 10 grams of mouse body weight. Note: 1ml i.p. injection of a nonirritating solution is easily tolerated. Position of animal: Manually restrained, dorsal recumbency (abdomen side up), with…

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