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Tag Archives: Immunohistochemistry

Common Fixatives Used for Immunohistochemistry

(1) 100% acetone. Pre-cold in a fridge. Cryosections are prepared, dry at room temperature overnight. Fixed with cold acetone for 20 min. (2) 4% paraformaldehyde in 0.1M phosphate buffer. Tissue can be cut into 3 × 3 × 6 mm in size; fixed for 12 h at room temperature. Dry cryosections can be fixed at…

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