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Tag Archives: E. coli Transformation

E. coli Transformation

Long 1) Get 200ul aliquots of E. coli (DH5a for normal transformation or DE3 for expression) from -80C freezer and let thaw on ice. 2) Add DNA For plasmid: 1ul DNA desired For ligation: 10ul ligation reaction -Also include a negative control with no DNA. 3) Incubate for 30min on ice. 4) Heat shock for…

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