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Tag Archives: DNA ligation protocol

DNA ligation

DNA ligation is the process of joining together two DNA molecule ends (either from the same or different molecules). Specifically, it involves creating a phosphodiester bond bond between the 3′ hydroxyl of one nucleotide and the 5′ phosphate of another. This reaction is usually catalyzed by a DNA ligase enzyme. This enzyme will ligate DNA fragments having blunt…

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