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Tag Archives: DNA extraction,blood samples

DNA extraction from blood samples  

  Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube. Expel blood immediately into a 1.5 ml microfuge tube containing 20 µl of 10 mM EDTA. Mix immediately to prevent clot formation. Store on ice until processing. Add 200 µl lysis buffer to each tube and vortex to suspend evenly. Microfuge…

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