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Tag Archives: Cell Cycle Analysis

Cell Cycle Analysis by PI Staining/FACS

Protocol 1. Fixation 1) Collect 1 X 106 cells after proper treatment. 2) Pellet cells by spinning at 2,000 rpm, 4C for 5 minutes. 3) Resuspend cell pellet in 1 ml of cold FCM buffer (5mM EDTA in 1xPBS) . 4) Fix cells by adding 1 ml of -20C absolute ethanol. 5) Store cells at -20C in…

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