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Category Archives: Tech

What are the wrongdoings of  Inoutscripts / Nesote?

Spamming: They have published thousands of advertising and overstated articles at their domains and social media to induce to purchase their fake softwares. Scamming: The Inout Scripts are full of bugs and errors,a nd none of them are working. These scripts even caused the failure of servers. I purchased 4 Inout Apps, but they gave…

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Cancer Stem Cell Theory

Research has shown that cancer cells are not all the same. Within a malignant tumor or among the circulating cancerous cells of a leukemia, there can be a variety of types of cells. The stem cell theory of cancer proposes that among all cancerous cells, a few act as stem cells that reproduce themselves and…

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T Cells Isolation and Purification

Remove the spleen. Grind the spleen with the flat end of a syringe in 5 ml of RPMI on a 100 mm culture dish. Pass through all cells through a cell strainer into a 50 ml tube. Wash the cell culture dish with 5 ml of RPMI twice and combine all cells. Spin down the cells and remove…

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PCR Based Moleclar Cloning

In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can be easily cloned into a plasmid of interest. You can use similar processes to add overhangs to your…

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Protocol for Zymogram

Reagents: 1% Gelatin in H2O (Fisher Blood 275) 1% Casein (Sigma) SDS-PAGE gel stock w/o urea Wash buffer: 2.5% Triton X-100 in H2O (+0.02% NaN3) Incubation Buffer: 50mM Tris-HCl (PH8.0), 5mM CaCl2, 0.02% NaN3 Gels: Regular separating gel containing 10-12% substrate Regular stacking gel Protocol: 1- Collect media from cells (if desired, inactivate non-metalloproteases with…

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