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Mutagenesis Protocols

‘Round-the-horn site-directed mutagenesis – OpenWetWare Categories: Protocol | DNA | In vitro … openwetware.org/wiki/’Round-the-horn_site-directed_mutagenesis   In vitro site-specific mutagenesis Human Molecular Genetics 2 conveys the feel of fast-moving research while providing a description in some depth of the techniques and data that are helping … www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg.section.611   Creating a deletion by PCR splicing This site…

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Mitochondrial DNA Isolation

What’s mitochondrial DNA? mitochondrial DNA (mtDNA) is a genetic material of the mitochondria, the organelles that generate energy for the cell. In mammals, mtDNA makes up less than 1% of the total cellular DNA, but in plants the amount is variable. It codes for ribosomal RNA and transfer RNA, but only some mitochondrial proteins (up to 30…

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Cell Cycle Analysis by PI Staining/FACS

Protocol 1. Fixation 1) Collect 1 X 106 cells after proper treatment. 2) Pellet cells by spinning at 2,000 rpm, 4C for 5 minutes. 3) Resuspend cell pellet in 1 ml of cold FCM buffer (5mM EDTA in 1xPBS) . 4) Fix cells by adding 1 ml of -20C absolute ethanol. 5) Store cells at -20C in…

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Single nucleotide polymorphisms (SNPs) and Databases

What are SNPs? Single nucleotide polymorphisms or SNPs (pronounced “snips”) are DNA sequence variations that occur when a single nucleotide (A,T,C,or G) in the genome sequence is altered. For example a SNP might change the DNA sequence AAGGCTAA to ATGGCTAA. For a variation to be considered a SNP, it must occur in at least 1%…

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Free eBooks of Biology and Biochemistry

A Brain for All Seasons — Human Evolution and Abrupt Climate Change A Stereotaxic Atlas of the Brain of the Zebra Finch Advances in Brain Imaging Alternative Medicine and Rehabilitation: A Guide for Practitioners Alzheimer’s Disease – Vaccine Prevents Alzheimer’s In Mice (applesforhealth) Amino Acid Properties Amphibians: Guidelines for the Breeding, Care and Management of…

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