Injection prompts mouse immune system to destroy tumors

All malignant tumors harbor genetic alterations. Some of these mutations lead to the production of modified proteins that can trigger an immune response. Tumors are often infiltrated by immune cells called T cells that recognize these altered proteins, or antigens. The field of cancer immunology aims to harness these immune responses. Several new methods of…

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Migration Assay on Endothelial Cells (ECs)

ECs, fed 24 hrs before experiment, 70-80% confluent at time of experiment . Transwells (8um, 6.5 mm, polycarbonate, Costar, cat #3422), coated on lowerside with 10 ug/ml fibronectin or vitronectin or other matrix in PBS (50 ul per well), overnight at 4oC or until dried (~2 hrs) in tissue culture hood. Control is coated with 0.5% fatty acids free…

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Real Time PCR Protocols

Real Time PCR vs. Traditional PCR Real Time PCR: A must read material Principal of Real Time PCR (60 slideshows) Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines Description Protocol 1. Quantitative Real Time PCR, a Practical Tutorial Protocol 2. Real time quantitative RT-PCR (Taqman) Protocol 3. TAQurate? GREEN Real-Time PCR MasterMix Real-time PCR complete solutions Protocol 4. Real…

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US to overtake Russia as top oil producer by 2019

The United States will overtake Russia as the world’s biggest oil producer by 2019 at the latest, the International Energy Agency (IEA) said on Tuesday, as the country’s shale oil boom continues to upend global markets. IEA Executive Director Fatih Birol said at an event in Tokyo the United States would overtake Russia as the…

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Bollywood star Sridevi Kapoor ‘drowned in hotel bath’

Superstar Bollywood actress Sridevi Kapoor died of accidental drowning, a Dubai post-mortem report has said. The 54-year-old actress, known simply as Sridevi, drowned in her hotel apartment’s bathtub following loss of consciousness, it says. It had earlier been reported that she died of cardiac arrest on Saturday while at a family wedding in Dubai. Bollywood…

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