Monthly Archives: February 2018

The Inoutscripts Scammer Replied me like this

I got a reply from the Inout Scripts Executive. Unfortunately, they did not admit they are phishing scammers and promise to revise these fake scripts, but falsely criticized my server and DNS. The fact about my server and DNS are as follows: They just overstated these Inoutscripts softwares to induce customers to buy. Finally, they…

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Injection prompts mouse immune system to destroy tumors

All malignant tumors harbor genetic alterations. Some of these mutations lead to the production of modified proteins that can trigger an immune response. Tumors are often infiltrated by immune cells called T cells that recognize these altered proteins, or antigens. The field of cancer immunology aims to harness these immune responses. Several new methods of…

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Migration Assay on Endothelial Cells (ECs)

ECs, fed 24 hrs before experiment, 70-80% confluent at time of experiment . Transwells (8um, 6.5 mm, polycarbonate, Costar, cat #3422), coated on lowerside with 10 ug/ml fibronectin or vitronectin or other matrix in PBS (50 ul per well), overnight at 4oC or until dried (~2 hrs) in tissue culture hood. Control is coated with 0.5% fatty acids free…

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Real Time PCR Protocols

Real Time PCR vs. Traditional PCR Real Time PCR: A must read material Principal of Real Time PCR (60 slideshows) Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines Description Protocol 1. Quantitative Real Time PCR, a Practical Tutorial Protocol 2. Real time quantitative RT-PCR (Taqman) Protocol 3. TAQurate? GREEN Real-Time PCR MasterMix Real-time PCR complete solutions Protocol 4. Real…

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US to overtake Russia as top oil producer by 2019

The United States will overtake Russia as the world’s biggest oil producer by 2019 at the latest, the International Energy Agency (IEA) said on Tuesday, as the country’s shale oil boom continues to upend global markets. IEA Executive Director Fatih Birol said at an event in Tokyo the United States would overtake Russia as the…

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