Monthly Archives: December 2017

Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing

Abstract: Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequencing results. Here, we describe a fast, cost-effective, and reliable method for preparation of mtDNA enriched samples from eukaryotic cells ready for direct…

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A Simplified Method for Mitochondrial DNA Extraction from Head Hair Shafts Featured

ABSTRACT: DNA isolation from hair shafts can involve a number of steps, each of which adds time to the procedure and increases the risk of contamination. A simple alkaline digestion procedure that directly dissolves hairs was developed and compared with a widely used glass grinding/organic extraction method, using samples collected from 30 volunteers with varying…

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Mitochondrial DNA Mutations and disease

Human mitochondrial DNA with groups of protein-, rRNA- and tRNA-encoding genes. The involvement of mitochondrial DNA in several human diseases. Susceptibility The concept that mtDNA is particularly susceptible to reactive oxygen species generated by the respiratory chain due to its proximity remains controversial. mtDNA does not accumulate any more oxidative base damage than nuclear DNA.…

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DNA ligation

DNA ligation is the process of joining together two DNA molecule ends (either from the same or different molecules). Specifically, it involves creating a phosphodiester bond bond between the 3′ hydroxyl of one nucleotide and the 5′ phosphate of another. This reaction is usually catalyzed by a DNA ligase enzyme. This enzyme will ligate DNA fragments having blunt…

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